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Monday, September 24, 2012

Plasmid Mapping and Arrays for all!

In Genetics today we continued our work on analyzing gels.  Today's task was to measure the DNA fragments bands on a gel to calculate their size in basepairs.  After these measurements, graphing the standard and extrapolation of fragment sizes from the standard (lambda cut with HindIII), we made a map of the cutting sites.  We also worked on previous AP mapping questions and our Chapter 6 discussion questions.  Tomorrow we will start our paper plasmid lab.

One of the plasmids we are looking at tomorrow is pBR322 ... shown below:

Structure of E.coli plasmid cloning vector pBR322

http://www.chemistrylearning.com/cloning-vector/

Selected Topics finished 7 days of weather, worked on plate tectonics and major events in geologic time.  Tomorrow is review day and the our earth science test will be Wednesday.

We assigned three single dimension array problems for this week in Computer Science ... Little League II, A gradebook program and a program to make address labels.  This will take almost all week.  After fall break, we return and spend some time doing graphics.

On the farm ... Rex shelled our corn today ... a tough year for all farmers.  My worse year farming ... 1981, we didn't start planting until late June and switched everything to soybeans after it rained all spring. A wet year and low commodity prices ... it was a nightmare!  Best part of 1981 ... our daughter was born!  I knew after that year, I didn't want to be held hostage by the weather and a few years later started teaching.


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