Big weather change here. Windy, rainy and colder, a reminder that winter will show up eventually. I am glad I made a last feeding of the bees yesterday.
I have bought enough wood to make some shims for my hives to winter feed our bees ... these shims are about 2.5 inches wide and will look like a very narrow super. Might be able to saw them out and put them together this weekend. There are lots of opinions about using shims ... the most notable is the imirie shim. I need something with more room for feedings.
http://www.brushymountainbeefarm.com/10-Frame-Imirie-Shim/productinfo/211/
Today as school ...
I found my Carolina shipment of live material today at school. So, I poured the agar plates and started our bacteria (E. coli) cultures this afternoon. Put the bacteria in our incubator for the night at 37 degrees C. Tomorrow we will run our student groups through a transformation procedure to induce a few of the bacteria to take in our plasmid (pBLU). This plasmid (small circular piece of DNA) has an antibiotic resistant gene (ampicillin) and a beta-galactosidase gene.
www.accessexcellence.org/AE/AEPC/SEP/pBLUteac.pdf
We will use ampicillin agar to sort out the bacteria that have taken up our plasmid. This is a two day lab. Will take some picts tomorrow.
In Selected Topics, we spent most of the class period balancing equations, part of our Chemistry unit. Tomorrow we will wrap up balancing equations and work on a Chemistry discovery timeline.
In Computer Programming, we are transitioning to Microsoft Visual BASIC, we will start checking our final 2-D array problems tomorrow.
Coach Carter and Coach Bunton will take softball practice tomorrow, I have a Science meeting from 3:00 - 5:00.
KP will be signing a letter of intent to play softball for WKU Friday at 2:00 PM. Will take some pictures and post them. Her grandfather was a custodian at our school for years ... I know he will be proud on Friday!
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